LP3IIG2細(xì)胞
ATCC Number： HB-8472?
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgG2a
LP3IIG2細(xì)胞Designations: LP3IIG2
Depositors: Genetic Systems Corp.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: LP3IIG2細(xì)胞immunoglobulin; monoclonal antibody; against Legionella pneumophila
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with Legionella pneumophila.
Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
LP3IIG2細(xì)胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 3893: Goldstein LC, Gosting LH. LP3IIG2細(xì)胞Method and composition for the detection and diagnosis of Legionella pneumophila. US Patent 4,851,333 dated Jul 25 1989
