12A5B7細胞
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG
數量： 大量
ATCC Number： HB-8328?
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
12A5B7細胞Designations: 12A5B7
Depositors: Immunomedics, Inc.
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 12A5B7細胞immunoglobulin; monoclonal antibody; against human complement C1q
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with purified human C1q.
12A5B7細胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3547: Reckel RP, et al. 12A5B7細胞Method for detecting immune complexes in serum. US Patent 4,595,654 dated Jun 17 1986
