19F8細(xì)胞
ATCC Number： HB-321?
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG2b; kappa light chain
生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
Designations: 19F8
Depositors: A Bendelac
19F8細(xì)胞Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591).
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse CD1.1
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 19F8細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected).
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody does not block recognition of CD1.1 by T cells.
The antibody reacts with CD1.1, and cross-reacts with CD1.2.
The epitope recognized by 19F8 is distinct from that recognized by the cluster represented by 15F7 (ATCC HB-322) and 20H2 (ATCC HB-323).
The antibody performs well in flow cytometry applications, but weakly in immunoprecipitation applications.
19F8細(xì)胞Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium.19F8細(xì)胞 Add medium as the cell density increases.
Maintain cultures at cell concentrations between 4 X 10(5) and 8 X 10(5) viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 38507: Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266
38508: Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267
38509: Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714
