Lec2細胞
細胞形態： 上皮樣
運輸方式： 凍存運輸
ATCC Number： CRL-1736?
是否是腫瘤細胞： 0
物種來源： 倉鼠
數量： 大量
器官來源： 卵巢
生長狀態： 單層（少量懸浮）
Lec2細胞Designations: Lec2 [originally named Pro-5WgaRII6A]
Depositors: P Stanley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer or suspension
Organism: Cricetulus griseus
Morphology: epithelial
Source: Organ: ovary
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Gender: female
Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.
The cells exhibit a drastic reduction in the transport of CMP-sialic acid into the Golgi compartment, and may be useful for studying the role of sialic acid in the function and compartmentalization of glycosylated macromolecules.
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 25 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
Propagation: Lec2細胞ATCC complete growth medium: Alpha minimum essential medium, 90%; fetal bovine serum, 10%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 22626: Deutscher SL, et al. Translocation across Golgi vesicle membranes: a CHO glycosylation mutant deficient in CMP-sialic acid transport. Cell 39: 295-299, 1984. PubMed: 6498937
58070: Stanley P, Siminovitch L. Complementation between mutants of CHO cells resistant to a variety of plant lectins. Somatic Cell Genet. 3: 391-405, 1977. Lec2細胞PubMed: 601679
