H9細胞

細胞形態： **樣

細胞類型： 其他細胞類型

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

生長狀態： 懸浮生長

年限： 53 years

數量： 大量

ATCC Number： HTB-176?

相關**： **瘤

Designations: H9 [derivative of HuT 78]

H9細胞Depositors: RC Gallo, M Popovic

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: lymphoma

Cell Type: cutaneous T lymphocyte;

Cellular Products: interleukin-2 (interleukin 2, IL-2)

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Applications:H9細胞 transfection host (Roche Transfection Reagents)

Receptors: interleukin 2 (IL-2)

Tumorigenic: Yes

Antigen Expression: CD4; HLA A1, B62, C3, DR4, DQ3

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,12

D16S539: 11,12

D5S818: 11

D7S820: 8,11

THO1: 8,9

TPOX: 8,9

vWA: 14,15

Cytogenetic Analysis: This is a near triploid cell line (modal number = 69; range = 58 to 74). The frequency of higher ploidies is 2.5%. The line has an extremely complex karyotype with nearly 60% of the chromosomes in each cell being structurally altered marker chromosomes., Among the markers are t(3p4q), t(5q6q), t(5p6p), i(18q), i(18p); t(4q7p), and del(7)(q32). The first four of these are usually paired. Normal N4, N5, N6, N7, N10, N13, N18, N19, N20 an X are absent.

H9細胞Isoenzymes: AK-1, 0

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 0

PGM1, 1

PGM3, 0

Age: 53 years

Gender: male

Ethnicity: Caucasian

Comments: The H9 cell line is a clonal derivative of the Hut 78 cell line (see ATCC TIB-161).

The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

H9細胞Do not allow cell concentration to exceed 3 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC TIB-161

References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

22948: Popovic M, et al. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497-500, 1984. PubMed: 6200935

23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

23367: Popovic M, et al. T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet 2: 1472-1473, 1984. PubMed: 6151082

29139: Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366

32558: Anderson SM, et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189