Hs 294T細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 皮膚

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： HTB-140?

相關(guān)**： 黑色素瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： 其他

年限： 56 years

Designations: Hs 294T

Depositors: RB Owens

Hs 294T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixed stellate and polygonal

Source: Organ: skin

Disease: melanoma

Derived from metastatic site: lymph node

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Receptors: nerve growth factor (NGF); interferon

Tumorigenic: Yes

Cytogenetic Analysis: Hs 294T細(xì)胞(P26) heteroploid with modal number = 59; Y chromosomes present

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

Me-2, 2

PGM1, 1-2

PGM3, 1

Age: 56 years

Gender: male

Ethnicity: Caucasian

Comments: The proliferative rate of the cell line is enhanced by addition of retinoic acid.

Responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon.

Hs 294T細(xì)胞Results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma.

ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 1125: Czarniecki CW, et al. Synergistic antiviral and anti-proliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. J. Virol. 49: 490-496, 1984. PubMed: 6319748

1126: Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934

2155: Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204

22590: Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522

22658: Creasey AA, et al. Hs 294T細(xì)胞Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563

26329: Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741