Y79細胞 
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		器官來源:	眼
	
	
		生長狀態:	懸浮生長
	
	
		ATCC Number:	HTB-18?
	
	
		數量:	大量
	
	
		相關**:	其他**
	
	
		細胞形態:	其他
	
	
		運輸方式:	凍存運輸
	
	
		組織來源:	retina
	
	
		年限:	2.5 years
	
	
		Designations:	Y79
	
	
		Depositors:	 B Gallie
	
	
		Y79細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	suspension
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	multicellular clusters
	
	
		
	
	
		Source:	Organ: eye
	
	
		Tissue: retina
	
	
		Disease: retinoblastoma
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Reverse Transcript:	negative
	
	
		DNA Profile (STR):	Amelogenin: X
	
	
		CSF1PO: 11,12
	
	
		D13S317: 11,12
	
	
		D16S539: 13,14
	
	
		D5S818: 11,12
	
	
		Y79細胞D7S820: 8,9
	
	
		THO1: 6,9.3
	
	
		TPOX: 8
	
	
		vWA: 15,18
	
	
		Cytogenetic Analysis:	hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes
	
	
		Isoenzymes:	AK-1, 1
	
	
		ES-D, 1
	
	
		G6PD, B
	
	
		GLO-I, 2
	
	
		PGM1, 1
	
	
		Age:	2.5 years
	
	
		Gender:	female
	
	
		Ethnicity:	Caucasian
	
	
		Comments: Y79細胞The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation.
	
	
		The donor had a strong maternal family history of retinoblastoma.
	
	
		Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
	
	
		Atmosphere: 5% CO2 in air recommended
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
	
	
		Medium Renewal: Twice per week
	
	
		Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks.
	
	
		Preservation:	Culture medium, 95%; DMSO, 5%
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	22531: Y79細胞Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597
	
	
		32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
	
	
		32684: Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545