AsPC-1細胞 
	
		生長狀態:	貼壁生長
	
	
		器官來源:	胰腺
	
	
		運輸方式:	凍存運輸
	
	
		ATCC Number:	CRL-1682?
	
	
		相關**:	腺癌
	
	
		年限:	62 years
	
	
		數量:	大量
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		Designations:	AsPC-1
	
	
		Depositors:	 MH Tan
	
	
		AsPC-1細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:
	
	
		Source:	Organ: pancreas
	
	
		Disease: adenocarcinoma
	
	
		Derived from metastatic site: ascites
	
	
		Cellular Products:	carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	transfection host
	
	
		DNA Profile (STR):AsPC-1細胞 Amelogenin: X
	
	
		CSF1PO: 10,13
	
	
		D13S317: 9,12
	
	
		D16S539: 11
	
	
		D5S818: 12
	
	
		D7S820: 12,13
	
	
		THO1: 7,9.3
	
	
		TPOX: 8,10
	
	
		vWA: 17
	
	
		Age:	62 years
	
	
		Gender:	female
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
	
	
		Propagation: AsPC-1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Protocol:
	
	
		Remove and discard culture medium.
	
	
		Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
	
	
		Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
	
	
		Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
	
	
		Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
	
	
		Add appropriate aliquots of the cell suspension to new culture vessels.
	
	
		Incubate cultures at 37?C.
	
	
		
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	2152: Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348
	
	
		22552: Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528
	
	
		22737: Loor R, et al. AsPC-1細胞Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226
	
	
		23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
	
	
		26140: Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565