NCI-H146細胞

生長狀態： 懸浮生長，多細胞聚集

細胞形態： 上皮樣

年限： 59 years

是否是腫瘤細胞： 1

物種來源： 人

器官來源： 肺

數量： 大量

ATCC Number： HTB-173?

運輸方式： 凍存運輸

相關**： 小細胞肺癌

Designations: NCI-H146 [H146]

NCI-H146細胞Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H146細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1979

Receptors: insulin-like growth factor II (IGF II)

Tumorigenic: Yes

Oncogene: myc +; myb +; raf +; ras +; fms +; fes -

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,12

D16S539: 11

D5S818: 12

D7S820: 9,10

THO1: 6,9.3

TPOX: 8,11

vWA: 14,16

Cytogenetic Analysis: This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. NCI-H146細胞At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 2

PGM1, 1-2

PGM3, 1-2

Age: 59 years

Gender: male

Ethnicity: Caucasian

Comments: The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.

The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified.

The cells do not express vasopressin, oxytocin or gastrin releasing peptide.

There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.

Propagation: NCI-H146細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

23065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

23162: Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

24389: . . Lung Cancer 4: 155-161, 1988.