Hs 445細胞
器官來源： **結
ATCC Number： HTB-146?
運輸方式： 凍存運輸
相關**： **瘤
年限： 56 years
是否是腫瘤細胞： 1
物種來源： 人
細胞形態： **樣
生長狀態： 懸浮生長
數量： 大量
Designations: Hs 445
Hs 445細胞Depositors: RB Owens
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: lymph node
Disease: Hodgkin's disease; Hodgkin's lymphoma
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,10.3
D13S317: 11,13
Hs 445細胞D16S539: 9,11
D5S818: 9,12
D7S820: 9,10
THO1: 6,7
TPOX: 8
vWA: 14,17
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments: Hs 445 was derived in 1970 from an intra-abdominal lymph node of a patient with stage 1 Hodgkin's disease.
By electron microscopy, herpesvirus-like particles have been seen.
0.3% to 0.5% of the cells are positive for EBV early antigen and virion antigen by immunofluorescence.
Propagation: Hs 445細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026
22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Hs 445細胞Cancer Inst. 65: 715-718, 1980. PubMed: 6932523
