SW1116細胞 
	
		年限:	Dukes' type A, grade III
	
	
		細胞形態:	上皮樣
	
	
		生長狀態:	貼壁生長
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		ATCC Number:	CCL-233?
	
	
		運輸方式:	凍存運輸
	
	
		數量:	大量
	
	
		器官來源:	結腸
	
	
		相關**:	大腸癌
	
	
		Designations:	SW1116 [SW 1116, SW-1116]
	
	
		Depositors:	 A Leibovitz
	
	
		SW1116細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: colon
	
	
		Tumor Stage: Dukes' type A, grade III
	
	
		Disease: colorectal adenocarcinoma
	
	
		Cellular Products:	carcinoembryonic antigen (CEA) 2654 ng/10 exp6 cells/10 days; keratin
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Tumorigenic:	Yes
	
	
		Oncogene:	myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -
	
	
		Antigen Expression:	blood type O; Rh+
	
	
		DNA Profile (STR): SW1116細胞Amelogenin: X,Y
	
	
		CSF1PO: 10,11
	
	
		D13S317: 11,14
	
	
		D16S539: 9,12
	
	
		D5S818: 11,12
	
	
		D7S820: 12
	
	
		THO1: 6
	
	
		TPOX: 8,11
	
	
		vWA: 14,19
	
	
		Cytogenetic Analysis:	modal number = 60; range = 50 to 62
	
	
		The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells.
	
	
		Isoenzymes:	ES-D, 1
	
	
		G6PD, B
	
	
		PEP-D, 1
	
	
		PGD, A
	
	
		PGM1, 1
	
	
		PGM3, 1-2
	
	
		Age:	73 years
	
	
		SW1116細胞Gender:	male
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	CSAp negative (CSAp-).
	
	
		Colon antigen 3, negative.
	
	
		The cells are positive for keratin by immunoperoxidase staining.
	
	
		The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.
	
	
		N-myc and N-ras oncogene expression were not detected.
	
	
		Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Atmosphere: air, 100%
	
	
		Subculturing:	Cells must be subcultured at about 80% confuency , before they reach 90%.
	
	
		Protocol: 1. Remove and discard culture medium.
	
	
		
	
	
		2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
	
	
		
	
	
		3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
	
	
		
	
	
		Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
	
	
		
	
	
		4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
	
	
		
	
	
		5. Add appropriate aliquots of the cell suspension to new culture vessels.
	
	
		
	
	
		6. Incubate cultures at 37?C.
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
	
	
		Medium Renewal: SW1116細胞1 to 2 times per week
	
	
		Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
	
	
		23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
	
	
		23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905