NCI-H510A細(xì)胞 
	
		生長狀態(tài):	混合型生長
	
	
		運(yùn)輸方式:	凍存運(yùn)輸
	
	
		數(shù)量:	大量
	
	
		器官來源:	肺
	
	
		細(xì)胞形態(tài):	上皮樣
	
	
		ATCC Number:	HTB-184?
	
	
		相關(guān)**:	其他**
	
	
		年限:	56 years
	
	
		是否是腫瘤細(xì)胞:	1
	
	
		物種來源:	人
	
	
		NCI-H510A細(xì)胞Designations:	NCI-H510A [H510A, NCI-H510]
	
	
		Depositors:	 AF Gazdar, JD Minna
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	mixed, adherent and suspension
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: lung
	
	
		Disease: carcinoma; small cell lung cancer; extrapulmonary origin
	
	
		Derived from metastatic site: adrenal gland
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H510A細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an ***** male patient.
	
	
		The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
	
	
		The line does not exhibit any gross structural DNA abnormalities.
	
	
		The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
	
	
		Tumorigenic:	Yes
	
	
		DNA Profile (STR):	Amelogenin: X
	
	
		CSF1PO: 10,12
	
	
		D13S317: 11,12
	
	
		D16S539: 13
	
	
		D5S818: 9,12
	
	
		THO1: 7,9.3
	
	
		TPOX: 8
	
	
		vWA: 15
	
	
		Cytogenetic Analysis: NCI-H510A細(xì)胞hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes were common to all cells. These included t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. Neither DM nor HSR were detected; structurally normal N1, N2, N13 and N21 were absent. Generally, there were 3 copies of both F group chromosomes; the X chromosomes were paired, and structurally normal Y chromosome was not found.
	
	
		Age:	56 years
	
	
		Gender:	male
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an ***** male patient.
	
	
		The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
	
	
		The line does not exhibit any gross structural DNA abnormalities.
	
	
		The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Protocol: This line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks.
	
	
		Subcultivation Ratio: NCI-H510A細(xì)胞A subcultivation ratio of 1:3 to 1:8 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Preservation:	Freeze medium: culture medium, 92.5%; DMSO, 7.5%
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		References:	1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
	
	
		23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257