MNNG/HOS (Cl #5)細(xì)胞 
	
		年限:	13 years
	
	
		是否是腫瘤細(xì)胞:	1
	
	
		物種來源:	人
	
	
		數(shù)量:	大量
	
	
		運(yùn)輸方式:	凍存運(yùn)輸
	
	
		器官來源:	骨
	
	
		ATCC Number:	CRL-1547?
	
	
		相關(guān)**:	骨肉瘤
	
	
		生長狀態(tài):	貼壁生長
	
	
		Designations:	MNNG/HOS Cl #5 [R-1059-D]
	
	
		Depositors:	 JS Rhim
	
	
		MNNG/HOS (Cl #5)細(xì)胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:
	
	
		Source:	Organ: bone
	
	
		Disease: osteosarcoma
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Tumorigenic:	Yes
	
	
		DNA Profile (STR):	Amelogenin: X
	
	
		CSF1PO: 12
	
	
		MNNG/HOS (Cl #5)細(xì)胞D13S317: 12
	
	
		D16S539: 10,13
	
	
		D5S818: 13
	
	
		D7S820: 11,12
	
	
		THO1: 6
	
	
		TPOX: 8,11
	
	
		vWA: 18
	
	
		Isoenzymes:	G6PD, B
	
	
		Age:	13 years
	
	
		Gender:	female
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
	
	
		Propagation: MNNG/HOS (Cl #5)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
	
	
		References:	22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046
	
	
		26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298
	
	
		32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. MNNG/HOS (Cl #5)細(xì)胞Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224