HEP G2/2.2.1細胞 
	
		數量:	大量
	
	
		ATCC Number:	CRL-11997?
	
	
		相關**:	肝癌
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		生長狀態:	貼壁生長
	
	
		器官來源:	肝
	
	
		年限:	15 years
	
	
		運輸方式:	凍存運輸
	
	
		細胞形態:	上皮樣
	
	
		Designations:	HEP G2/2.2.1
	
	
		HEP G2/2.2.1細胞Depositors:	 Northeastern Ohio University College of Medicine
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens deposited as human
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: liver
	
	
		Disease: hepatocellular carcinoma
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		DNA Profile (STR):	Amelogenin: X,Y
	
	
		CSF1PO: 10,11
	
	
		D13S317: 9,13
	
	
		D16S539: 12,13
	
	
		HEP G2/2.2.1細胞D5S818: 11,12
	
	
		D7S820: 10
	
	
		THO1: 9
	
	
		TPOX: 8,9
	
	
		vWA: 17
	
	
		Age:	15 years
	
	
		Gender:	male
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	Cell line was derived from the hepatocellular carcinoma cell line, HepG2 (ATCC HB-8065). The parental cells were stably transfected at passage 48 with a human cholesterol 7 alpha-hydroxylase (CYP7) minigene/Luciferase construct.
	
	
		Propagation:	ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate and 1200 mg/L sodium bicarbonate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Temperature: 37.0°C
	
	
		Growth Conditions: HEP G2/2.2.1細胞These cells are slow to attach after subculture. Allow 4 to 5 days for reattachment.
	
	
		Subculturing:	Protocol:
	
	
		Remove and discard culture medium.
	
	
		Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
	
	
		Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
	
	
		Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
	
	
		Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
	
	
		Add appropriate aliquots of the cell suspension to new culture vessels.
	
	
		Incubate cultures at 37?C.
	
	
		
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
	
	
		Medium Renewal: Every 2 to 3 days
	
	
		Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
	
	
		recommended serum:ATCC 30-2020
	
	
		parental cell line:ATCC HB-8065
	
	
		References:	40153: Chiang JY, Stroup D. HEP G2/2.2.1細胞Assay for agents that affect cholesterol 7alpha-hydroxylase expression and a characterization of its regulatory elements. US Patent 5,821,057 dated Oct 13 1998