RPMI-7951細(xì)胞 
	
		細(xì)胞形態(tài):	上皮樣
	
	
		生長狀態(tài):	貼壁生長
	
	
		數(shù)量:	大量
	
	
		ATCC Number:	HTB-66?
	
	
		相關(guān)**:	惡性黑色素瘤
	
	
		年限:	18 years
	
	
		是否是腫瘤細(xì)胞:	1
	
	
		物種來源:	人
	
	
		器官來源:	皮膚
	
	
		運(yùn)輸方式:	凍存運(yùn)輸
	
	
		Designations:	RPMI-7951
	
	
		Depositors:	 G Moore
	
	
		RPMI-7951細(xì)胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: skin
	
	
		Disease: malignant melanoma
	
	
		Derived from metastatic site: lymph node
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Isolation:	Isolation date: 1971
	
	
		Tumorigenic:	Yes
	
	
		Antigen Expression:	Blood Type A; Rh+
	
	
		DNA Profile (STR): RPMI-7951細(xì)胞Amelogenin: X
	
	
		CSF1PO: 12
	
	
		D13S317: 11,12
	
	
		D16S539: 11,12
	
	
		D5S818: 11
	
	
		D7S820: 11,12
	
	
		THO1: 9,9.3
	
	
		TPOX: 8
	
	
		vWA: 17,19
	
	
		Cytogenetic Analysis:	modal number= 49; range = 47 to 66.
	
	
		This is a hyperdiploid human cell line with the modal chromosome number of 49, occurring in 24% of cells. Polyploid cells occurred at 22%, which is high. Seven marker chromosomes were common to most cells, including t(10p14q); t(15q17p), t(Xp8p), del(17) (p13.1) and three others. Five others occurred only in some cells. Normal X chromosomes were absent. N14, N17 and N22 were mostly single-copied and N2 had three copies. Fluorescence examination did not show the presence of any Y-like chromosome.
	
	
		Isoenzymes:	AK-1, 1
	
	
		ES-D, 1
	
	
		G6PD, B
	
	
		GLO-I, 1
	
	
		PGM1, 1-2
	
	
		PGM3, 1
	
	
		Age:	18 years
	
	
		Gender:	female
	
	
		Ethnicity: RPMI-7951細(xì)胞Caucasian
	
	
		Comments:	A contaminant identified as Mycoplasma fermentans was eliminated in 1975.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Protocol:
	
	
		Remove and discard culture medium.
	
	
		Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
	
	
		Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
	
	
		Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
	
	
		Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
	
	
		Add appropriate aliquots of the cell suspension to new culture vessels.
	
	
		Incubate cultures at 37?C.
	
	
		
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
	
	
		Medium Renewal: Two to three times weekly
	
	
		Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	22536: RPMI-7951細(xì)胞Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
	
	
		22540: . . J. Natl. Cancer Inst. 59: 301-307, 1977.
	
	
		25093: Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479