SKO-007細(xì)胞 
	
		運(yùn)輸方式:	凍存運(yùn)輸
	
	
		細(xì)胞類型:	B**細(xì)胞
	
	
		是否是腫瘤細(xì)胞:	1
	
	
		物種來源:	人
	
	
		ATCC Number:	CRL-8033-1?
	
	
		生長狀態(tài):	懸浮生長
	
	
		數(shù)量:	大量
	
	
		細(xì)胞形態(tài):	**樣
	
	
		**類型:	IgE; lambda light chain
	
	
		Designations:	SKO-007
	
	
		Depositors:	 H Kaplan, Stanford University Board of Trustees
	
	
		SKO-007細(xì)胞Isotype:	IgE; lambda light chain
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	suspension
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	lymphoblast
	
	
		
	
	
		Source:	Cell Type: B lymphocyte;
	
	
		Cellular Products:	immunoglobulin; monoclonal antibody
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Antigen Expression:	HLA A2, A3, B7, Bw60, Bw6, Cw3
	
	
		DNA Profile (STR):	Amelogenin: X,Y
	
	
		CSF1PO: 12,13
	
	
		D13S317: 12
	
	
		SKO-007細(xì)胞D16S539: 10
	
	
		D5S818: 11,12
	
	
		D7S820: 11,12
	
	
		THO1: 5,7
	
	
		TPOX: 8
	
	
		vWA: 17
	
	
		Comments:	This line was derived from the U266 myeloma cell line of Nilsson (Clin. Exp. Immunol. 7:477-489, 1970) by selecting for resistance to 8-azaguanine;the cells cells are HAT sensitive.
	
	
		This is a batch of SKO-007 SKO-007細(xì)胞(formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination.
	
	
		The J3 clone of SKO-007 is available also (ATCC CRL-8033-2).
	
	
		Propagation:	ATCC complete growth medium: RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Medium Renewal: 2 to 3 times per week
	
	
		Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
	
	
		Related Products:	parental cell line:ATCC CRL-8033
	
	
		References:	1214: Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. SKO-007細(xì)胞PubMed: 6159646