COLO 205細胞 
	
		年限:	Dukes' type D
	
	
		數量:	大量
	
	
		ATCC Number:	CCL-222?
	
	
		相關**:	大腸癌
	
	
		細胞形態:	上皮樣
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		運輸方式:	凍存運輸
	
	
		生長狀態:	混合型生長
	
	
		器官來源:	結腸
	
	
		Designations:	COLO 205
	
	
		Depositors:	 GE Moore
	
	
		COLO 205細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	mixed, adherent and suspension
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: colon
	
	
		Tumor Stage: Dukes' type D
	
	
		Disease: colorectal adenocarcinoma
	
	
		Derived from metastatic site: ascites
	
	
		Cellular Products:	carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	transfection host
	
	
		Tumorigenic:	Yes
	
	
		DNA Profile (STR): COLO 205細胞Amelogenin: X
	
	
		CSF1PO: 11,12
	
	
		D13S317: 10,12
	
	
		D16S539: 12,13
	
	
		D5S818: 10,13
	
	
		D7S820: 9,10
	
	
		THO1: 8,9
	
	
		TPOX: 11
	
	
		vWA: 15
	
	
		Cytogenetic Analysis:	The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
	
	
		Isoenzymes:	ES-D, 1-2
	
	
		G6PD, B
	
	
		PEP-D, 1
	
	
		PGD, A
	
	
		PGM1, 1-2
	
	
		PGM3, 1-2
	
	
		Age:	70 years
	
	
		Gender:	male
	
	
		Ethnicity: COLO 205細胞Caucasian
	
	
		Comments:	This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
	
	
		The cells are CSAp negative (CSAp-).
	
	
		The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224).
	
	
		The cells are positive for keratin by immunoperoxidase staining.
	
	
		COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Protocol: Shake flask, transfer floating cells inot a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
	
	
		Medium Renewal: Every 2 to 3 days
	
	
		Preservation:	Freeze medium: COLO 205細胞Complete culture medium, 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
	
	
		recommended serum:ATCC 30-2020
	
	
		derived from same individual:ATCC CCL-224
	
	
		References:	1046: Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251
	
	
		22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757
	
	
		22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
	
	
		41500: Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697