SW1417細(xì)胞
器官來源： 結(jié)腸
細(xì)胞形態(tài)： 上皮樣
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 1
物種來源： 人
生長狀態(tài)： 貼壁生長
年限： Dukes' type C, grade III
ATCC Number： CCL-238?
數(shù)量： 大量
相關(guān)**： 大腸癌
Designations: SW1417 [SW-1417]
Depositors: A Leibovitz
SW1417細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes' type C, grade III
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 362 ng/10 exp6 cells/10 days; keratin
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Tumorigenic: No
Oncogene: myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -
Antigen Expression: blood type B; Rh+
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1
Age: 53 years
Gender: female
Ethnicity: SW1417細(xì)胞Caucasian
Comments: CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.
N-myc, K-ras, N-ras and myb oncogene expression were not detected.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. SW1417細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
23074: Dahiya R, et al. SW1417細(xì)胞ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
