SW 982細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
ATCC Number： HTB-93?
相關(guān)**： 其他**
年限： 25 years
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
細(xì)胞形態(tài)： 混合型
組織來(lái)源： synovium
Designations: SW 982 [SW-982, SW982]
SW 982細(xì)胞Depositors: A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: mixed
Source: Tissue: synovium
Disease: synovial sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
THO1: 9.3
SW 982細(xì)胞TPOX: 9,11
vWA: 19,20
Cytogenetic Analysis: Hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%. Nine markers were common to all cells. These were: t(1q4p), del(5) (q31;q33), der(9) t(4;9) (q11;p24), t(8q12p), t(9q13q) and four others.Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1-2
Age: 25 years
Gender: female
Ethnicity: Caucasian
Comments: The SW 982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian.
The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma.
An ampule at passage 4 was received at the ATCC in January, 1982.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. SW 982細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. SW 982細(xì)胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
