MC116細胞
運輸方式： 凍存運輸
細胞類型： B**細胞
是否是腫瘤細胞： 1
物種來源： 人
組織來源： ascites
ATCC Number： CRL-1649?
相關**： 其他**
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgM; lambda light chain
數量： 大量
Designations: MC116
MC116細胞Depositors: IT Magrath
Isotype: IgM; lambda light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: ascites
Disease: undifferentiated lymphoma
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. MC116細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: HLA A1, A3, B8, Bw35
Cytogenetic Analysis: 45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)
Gender: male
Comments: The cells are EBNA negative, negative for Fc receptors and negative for complement receptors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 10 exp5 viable cells/ml and maintain at or below 3 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 26 to 28 hrs
Related Products: MC116細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22548: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. MC116細胞PubMed: 6243722
