SK-CO-1細胞
器官來源： 結腸
運輸方式： 凍存運輸
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： HTB-39?
相關**： 大腸癌
細胞形態(tài)： 上皮樣
年限： 65 years
生長狀態(tài)： 貼壁生長
Designations: SK-CO-1
Depositors: G Trempe, LJ Old
SK-CO-1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: colorectal adenocarcinoma
Derived from metastatic site: ascites
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) SK-CO-1細胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -
Antigen Expression: Blood Type O; Rh+; HLA A1, A3, B7, B13
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 14
D16S539: 13
D5S818: 9,11
D7S820: 8,10
THO1: 8,9
TPOX: 8,11
vWA: 18,19
SK-CO-1細胞Cytogenetic Analysis: (P7) hypertriploid to hypotetraploid with abnormalities including dicentrics, minutes, rings, secondary constrictions, and 8 large submetacentric markers
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1-2
Age: 65 years
Gender: male
Ethnicity: Caucasian
Comments: Ultrastructural features include microvilli, microfilaments, small mitochondria with dark granules and dilated cristae, sparse RER, few Golgi, lipid droplets, multilamellar bodies, no virus particles were detected.
The line is positive for expression of c-myc, N-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: SK-CO-1細胞Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066
32794: Kutchera W, et al. SK-CO-1細胞Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486
