182-PF SK細胞
ATCC Number： CRL-1532?
相關**： 其他**
運輸方式： 凍存運輸
數量： 大量
細胞形態： 成纖維樣
是否是腫瘤細胞： 1
物種來源： 人
年限： 20 years
器官來源： 皮膚
182-PF SK細胞生長狀態： 貼壁生長
Designations: 182-PF SK
Depositors: L Kopelovich
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: hereditary adenomatosis
Permits/Forms: 182-PF SK細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Caucasian
Comments: The tissue donor suffered from hereditary adenomatosis.
The line is capable of 10 population doublings after thawing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: 182-PF SK細胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
References: 22736: Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781
22759: . . Prog. 182-PF SK細胞Cancer Res. Ther. 13: 97-108, 1980.
