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                產(chǎn)品名稱(chēng): pCOLADuet-1 
            
            
                產(chǎn)品型號(hào): 
            
                產(chǎn)品展商: HZbscience
            
            
            
            
                產(chǎn)品文檔: 無(wú)相關(guān)文檔
            
            
            
            
                簡(jiǎn)單介紹
            
            
                pCOLADuet-1的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問(wèn)題,請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到pCOLADuet-1后請(qǐng)短暫離心,取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中,挑取單克隆重新提取質(zhì)粒后使用。
            
            
                pCOLADuet-1
                 的詳細(xì)介紹
            
        
            
	pCOLADuet-1載體基本信息 
	
		
			
				
					
						
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							pCOLADuet1, pCOLADuet 1
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							大腸桿菌蛋白雙表達(dá)載體,有兩個(gè)多克隆位點(diǎn)
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							高
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							多克隆位點(diǎn),限制性內(nèi)切酶
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							3719 bp
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							T7
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							T7: 5'-TAATACGACTCACTATAGGG-3';
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							T7t
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							T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
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							N-His, C-S
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							Kanamycin
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								pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
							 
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							71406-3
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							瞬時(shí)表達(dá) Transient
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							組成型 Constitutive
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							非病毒
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		pCOLADuet-1載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
	
	
	
		pCOLADuet-1載體簡(jiǎn)介
	
	
		
			
				pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His?Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S?Tag? peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
			
		 
	 
	
		pCOLADuet-1載體序列
	
hz-4587R	IL-6  白介素6抗體
hz-4588R	IGF I  胰島素樣生長(zhǎng)因子1抗體
hz-4590R	PPAR Gamma  過(guò)氧化酶活化增生受體γ抗體
hz-4210R	C9orf156  9號(hào)染色體開(kāi)放閱讀框156抗體
hz-4592R	P14ARF/CDKN2A  抑癌基因p16抗體
hz-4593R	MMP9  基質(zhì)金屬蛋白酶9抗體
hz-4600R	TIMP-1(CT)  金屬蛋白酶組織抑制因子-1抗體(C端)
hz-1114R	Adducin protein  內(nèi)收蛋白抗體
hz-4601R	phospho-c-Jun(Thr91+Thr93)  磷酸化原癌基因c-Jun抗體
hz-4606R	Gibberellins  抗赤霉素抗體
hz-4610R	phospho-AQP2(Ser264/261)  磷酸化水通道蛋白2抗體
hz-4622R	Mycobacterium bovis  牛結(jié)核桿菌菌體蛋白抗體
hz-4625R	CK12/Cytokeratin 12  細(xì)胞角蛋白12抗體
hz-4626R	HE4/Epididymal secretory protein E4  附睪分泌蛋白4抗體
hz-4631R	Beta galactosidase  β半乳糖苷酶抗體
hz-4636R	NR2C2/TAK1  孤兒核受體TAK1抗體
hz-4642R	Kilham Rat Virus/KRV-VP1/KRV-VP2 (C-terminus)  基爾曼氏細(xì)小病毒VP1/VP2抗體(大鼠潛在病毒KRV)C端
hz-4643R	Kilham Rat Virus/KRV-VP1/KRV-VP2 (C-terminus)  基爾曼氏細(xì)小病毒VP1/VP2抗體(大鼠潛在病毒KRV)C端
hz-4646R	Capsid protein VP1  大鼠細(xì)小病毒H-1株(H-1)抗體(N端)