pREP4 載體描述 These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.
pREP4 is an episomal mammalian expression vector that uses the Rous Sarcoma Virus long terminal repeat (RSV LTR) enhancer/promoter for transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by the plasmid to permit extrachromosomal replication in human, primate and canine cells. pREP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pREP4/CAT is provided as a control for the relative level of expression of recombinant proteins in a cell line of interest. pREP4/CAT expresses the chloramphenicol acetyl transferase protein from the RSV LTR enhancer/promoter. pREP4/CAT contains the hygromycin B resistance gene for selection.