Product Description
Versions of the pFLAG-CMV-5 expression vector for the three translational reading frames preceding the FLAG coding sequence are provided.

pFLAG-CMV-5a expression vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of C-terminal FLAG fusion proteins in mammalian cells.

The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs.
The multiple cloning region of the pFLAG-CMV-5a expression vector is not preceded by a translational initiation signal. A translational start sequence3 must be included with the insert DNA.
pFLAG-CMV-5a expression vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.


p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.