The pEF6/V5-His TOPO TA Expression Kit is designed for high-level mammalian 
expression from the powerful human EF-1 α promoter with the added convenience 
of one-step TOPO Cloning. The pEF6/V5-His-TOPO vector includes:

Enhancer/promoter elements from the human elongation factor 1α 
subunit (hEF-1α) for high-level expression in mammalian cells

 The Blasticidin resistance gene for rapid selection of stable 
mammalian cell lines
 A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
 A C-terminal polyhistidine (6xHis) sequence for rapid purification 
with nickel-chelating resin and detection with an Anti-His(C-term) Antibody