The pShooter vectors are designed to localize recombinant proteins to the nucleus, mitochondria, 

endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter vector 

contains:

? Strong mammalian promoter (CMV or EF-1a) for constitutive expression of your protein of interest
? C-terminal c-myc epitope for detection with an Anti-myc Antibody
? Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability
? f1 origin for single-stranded rescue of sense DNA
? Neomycin resistance gene for selection of mammalian cells with Geneticin selection agent
? Ampicillin resistance and pUC origin for selection and maintenance in E. coli

Each vector is provided with a positive control plasmid. The positive control expresses GFP and targets 

it to the same subcellular location as the pShooter vector.