The InsectSelect Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:

? The OpIE2 promoter for constitutive expression
? The Zeocin resistance gene for rapid selection of stably transfected cell lines
? C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells.

Promoter:	OplE2
Vector Type:	pIZ
Product Size:	1 kit
Cloning Method:	Restriction Enzyme ? MCS
Protein Tag or Fusion:	V5 Epitope Tag, His Tag (6x)
Protein Tag Position (to your gene):	C-terminal