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pXINSECT-DEST39

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產品名稱: pXINSECT-DEST39
產品型號:
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

pXINSECT-DEST39的各批次質粒菌株發貨前均經過嚴格的多重驗證,如存在質量問題,請在收到產品的三個月內通知我司。收到pXINSECT-DEST39后請短暫離心,取2μl轉化至對應感受態中,挑取單克隆重新提取質粒后使用。


pXINSECT-DEST39  的詳細介紹

pXINSECT-DEST39載體基本信息

載體名稱: pXINSECT-DEST39
質粒類型: 昆蟲表達載體;Gateway載體
克隆方法: Gateway
啟動子: Actin
載體大小: 12447 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: 6XHis tag (N-末端)
載體抗性: 氨芐青霉素氯霉素(僅空載體)
真核篩選標記:
克隆菌株: DB3.1
宿主細胞(系): High Five(推薦) 等細胞系
備注: pXINSECT-DEST39載體與pBmA-neo載體共轉染,可以建立穩表達細胞系;
應用Gateway技術,可快速高效將 目的基因片段(尤其適用于目的基因片段>1kb,用傳統的連接酶法效率低的)插入到載體中;
產品目錄號: 12249-025
瞬表達/穩表達: 瞬表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒

pXINSECT-DEST39載體質粒圖譜和多克隆位點信息

pXINSECT-DEST39 載體圖譜



pXINSECT-DEST39 多克隆位點

pXINSECT-DEST39 載體特征

pXINSECT-DEST39載體簡介

pXINSCET-DEST39載體優勢: Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
 Non-lytic, continuous protein expression
 Minimal degradation of your protein of interest as compared to viral expression systems
 Higher protein expression level achieved than other similar non-viral insect expression systems
 Choice of transient or stable protein expression
 Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning pXINSCET-DEST39載體特征: The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
 Chloramphenicol resistance gene located between the two attR sites for counterselection
 The ccdB gene located between the two attR sites for negative selection
 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
 Ampicillin resistance gene for selection of transformants in E. coli
 The pUC origin for high copy replication and maintenance of the plasmid in E. coli 

pXINSECT-DEST39載體序列

hz-5183R phospho-c-Abl(Thr754)  磷酸化非受體酪氨酸激酶c-Abl抗體
hz-2940R FUCA1/Alpha L fucosidase I  α-L巖藻糖苷酶抗體
hz-2941R TNFR1/TNF Receptor I  腫瘤壞死因子受體1抗體
hz-2942R HHV8/ORF K14  人類皰疹病毒8 ORF14抗體
hz-2943R ShzA6D  軸突導向因子ShzA6D抗體
hz-2944R plant lectin B4/IB4  植物凝集素IB4
hz-2945R SNX1/Sorting nexin1  分選連接蛋白1抗體
hz-2947R ERG25  甲基固醇羥化酶單加氧酶1抗體
hz-5184R phospho-c-Abl(Tyr283)  磷酸化非受體酪氨酸激酶c-Abl抗體
hz-2950R Cytosine deaminase  胞嘧啶脫氨酶抗體
hz-2951R SQSTM1/p62  泛素結合蛋白P62抗體
hz-2952R NFAT4  核因子活化T細胞胞漿蛋白3抗體
hz-2953R Exportin 1/CRM1  染色體區域穩定蛋白/核輸出蛋白1/染色體區域穩定必需蛋白抗體
hz-2954R IKZF3  鋅指蛋白Aiolos抗體
hz-2955R C3a Receptor/C3aR  過敏**C3受體/補體C3受體抗體
hz-2957R Alpha-ENaC  鈉通道蛋白α ENaC
hz-2958R PLAGL2  多形性腺瘤基因樣蛋白2抗體
hz-2959R 5HTR2C  5-羥色胺受體2C抗體

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