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pXINSECT-DEST38

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產品名稱: pXINSECT-DEST38
產品型號:
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

pXINSECT-DEST38的各批次質粒菌株發貨前均經過嚴格的多重驗證,如存在質量問題,請在收到產品的三個月內通知我司。收到pXINSECT-DEST38后請短暫離心,取2μl轉化至對應感受態中,挑取單克隆重新提取質粒后使用。


pXINSECT-DEST38  的詳細介紹

pXINSECT-DEST38載體基本信息

載體名稱: pXINSECT-DEST38
質粒類型: 昆蟲表達載體;Gateway載體
克隆方法: Gateway
啟動子: Actin
載體大小: 12419 kb
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: 無標簽
載體抗性: 氨芐青霉素氯霉素(僅空載體)
真核篩選標記:
克隆菌株: DB3.1
宿主細胞(系): High Five(推薦) 等細胞系
備注: pXINSECT-DEST38載體與pBmA-neo載體共轉染,可以建立穩表達細胞系;
應用Gateway技術,可快速高效將 目的基因片段(尤其適用于目的基因片段>1kb,用傳統的連接酶法效率低的)插入到載體中;
產品目錄號: 12249-025
瞬表達/穩表達: 瞬表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒

pXINSECT-DEST38載體質粒圖譜和多克隆位點信息

pXINSECT-DEST38 載體圖譜



pXINSECT-DEST38 多克隆位點

pXINSECT-DEST38 載體特征

pXINSECT-DEST38載體簡介

 pXINSECT-DEST38載體優勢:
Using the Express Insect Kit or the Express Insect Vector Set to achieveprotein expression in insect cells has the following advantages:
 Non-lytic, continuous protein expression
 Minimal degradation of your protein of interest as compared to viralexpression systems
 Higher protein expression level achieved than other similar non-viral insectexpression systems
 Choice of transient or stable protein expression
 Adapted for use with the Gateway Technology or traditional restrictionenzyme-mediated cloning

載體特征:
pXINSECT-DEST38 and pXINSECT-DEST39 have the following features:
 The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
 Chloramphenicol resistance gene located between the two attR sites for counterselection
 The ccdB gene located between the two attR sites for negative selection
 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
 Ampicillin resistance gene for selection of transformants in E. coli
 The pUC origin for high copy replication and maintenance of the plasmid in E. coli 

pXINSECT-DEST38載體序列

hz-2961R Calsequestrin  肌鈣集蛋白/收鈣素抗體
hz-2962R Syncytin 1/HERV  合胞素1抗體
hz-2963R TASK 3/KCNK9  酸敏感性鉀離子通道蛋白抗體
hz-2964R TSLP  胸腺基質**細胞生成素-1抗體
hz-2965R Myosin-XVIIIb  肌球蛋白XVIIIb抗體
hz-2966R RBPJK/RBP-J  Notch轉錄調控蛋白RBPJK抗體
hz-2967R Maltose Binding Protein/MBP  麥芽糖結合蛋白抗體
hz-2968R HPV33 E6  人類**狀瘤病毒33抗體
hz-2969R HPV33 E7  人類**狀瘤病毒33抗體
hz-2970R PHGDH  磷酸甘油酸脫氫酶抗體
hz-2971R PASK/STK39  絲氨酸/蘇氨酸蛋白激酶39抗體
hz-2972R HES1  轉錄因子HES-1抗體
hz-2974R Factor VIII  第八因子抗體
hz-2975R Noggin  指(趾)關節粘連NOG蛋白抗體
hz-2976R GGT1/CD224  γ谷氨酰轉移酶抗體
hz-2977R TAGL2/TAGLN2  細胞骨架相關蛋白2抗體
hz-2978R Peroxiredoxin 2/PRXII  硫氧還蛋白過氧化物酶Ⅱ/巰基抗氧化蛋白抗體
hz-2981R IL-8/CXCL8  白介素8抗體
hz-2982R VIP Receptor 1/VAPC1  血管活性腸肽受體-1抗體
hz-2983R Desmocollin 2  橋粒糖蛋白2/橋粒糖蛋白3抗體
hz-2984R phospho-PLB(Ser16)  磷酸化心臟磷蛋白抗體
hz-2985R ADM2  中介素抗體
hz-2986R CATSPER  陽離子通道精子相關蛋白1抗體
hz-2987R Vitamin D Receptor/VDR  維生素D3受體抗體

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