The pIEx/Bac-3 vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in 

Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) 

enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV 

p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions 

to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His?Tag® coding sequence with 10 

histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal 

thrombin protease cleavage site and 8 aa Strep?Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 

3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for 

amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.