The pcDNA vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:
Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
C-terminal V5 tag for easy detection
Neomycin resistance gene for stable selection
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate ORF Clone. The following table lists a variety of available destination vectors.
Gateway哺乳動物表達載體選擇指南：
pDEST12.2 不帶標簽
pDEST26 N-His標簽 G418篩選
pDEST27 N-GST標簽 G418篩選
pDEST40 C-His標簽、C-V5標簽 G418篩選
pcDNA-DEST40 C-His標簽、C-V5標簽 G418篩選
pcDNA-DEST47 C-綠色熒光蛋白 G418篩選
pcDNA-DEST53 N-綠色熒光蛋白 G418篩選
pcDNA3.2/V5-DEST C-V5標簽 G418篩選
pcDNA6.2/V5-DEST C-V5標簽 Blasticidin S篩選
pcDNA6.2/N-YFP-DEST N-黃色熒光蛋白 Blasticidin S篩選
pcDNA6.2/N-EmGFP-DEST N-綠色熒光蛋白 Blasticidin S篩選
pcDNA6.2/C-YFP-DEST C-黃色熒光蛋白 Blasticidin S篩選
pcDNA6.2/C-EmGFP-DEST C-綠色熒光蛋白 Blasticidin S篩選
pcDNA6.2/BioEase-DEST N-生物素 Blasticidin S篩選
