The pcDNA vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway pcDNA-DEST47 vector offers the following key features:

C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
Neomycin resistance gene for stable selection
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate ORF Clone. The following table lists a variety of available destination vectors.