Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to c
reate a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector
to create an expression clone.
The Gateway entry vectors offer the following:
? attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination
vector to ensure cloning of the gene of interest in the correct orientation for expression
? Kozak consensus sequence for efficient translation initiation in eukaryotic systems
? Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR? 1A Dual
Selection, pENTR?3C Dual Selection, and pENTR?11 Dual Selection vectors only)
? rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
? pUC origin for high-copy replication and maintenance of the plasmid in E. coli
? Kanamycin resistance gene for selection in E. coli
? The ccdB?chloramphenicol fusion gene located between the two attL sites for negative selection and
o Chloramphenicol selection in E. coli
? Kanamycin resistance gene for selection in E. coli
