pRI909載體簡介：
Binary Vectors for Agrobacterium-mediated Plant Transformation: pRI909 and pRI910
Ri Plasmid pRI909 and pRI910 are binary vectors intended for Agrobacterium-mediated plant
transformation and contain a region of T-DNA for Arabidopsis, tomato, tobacco and rice
transformation. The origin of the T-DNA is the vir region-lacking Rhizobium (Agrobacterium)
rhizogenes Ri plasmid. Ri Plasmid pRI909 and pRI910 are also shuttle vectors and replicate
autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are capable
of high copy number because they contain the ColE1 replication origin. The ColE1 ori, as well
as the mutant-type Ri plasmid replication of origin (Ri-ori), are each stably maintained in
Rhizobium (Agrobacterium). These vectors also contain the kanamycin resistance gene NPTIII
for selection in E. coli and Rhizobium (Agrobacterium) and a mutant-type kanamycin resistance
gene NPTII for selection in plants.
The Ri Plasmid pRI909 and pRI910 vectors are capable of plant transformation in combination
with Rhizobium (Agrobacterium) through a binary vector system. Stable plant chromosome target
gene integration is possible with the Ri Plasmid pRI909 and pRI910 because their cloning
sites are located in close proximity to the T-DNA Right Border(RB) and not the NPT II plant
selection marker. As a result, the target gene is not deleted.
pRI909載體特征：
Easy and stable plant chromosome target gene integration
Improved agrobacterium-mediated plant transformation
Versatile: Applicable to various plants such as Arabidopsis, tomato, tobacco, and rice
Applications
Rhizobium (Agrobacterium)-mediated plant transformation
Arabidopsis, tomato, tobacco, rice transformation
Purity
>70% double-stranded covalently closed circular form I (RFI) DNA.
Cloning sites verified by dideoxy DNA sequencing.
Restriction sites verified by restriction enzyme cleavage.
參考文獻：
1. Nishiguchi, R. et al. (1987) Mol. Gen. Genet. 206:1-8.
2. Ooms, G. et al. (1982) Plasmid 7:15-29.
3. Ohba, T. et al. (1995) The Plant J. 7:157-164.
