Product Name	Rabbit anti-RELA polyclonal antibody - middle region
Antibody Type	Primary Antibodies
Immunogen	The immunogen for anti-RELA antibody: synthetic peptide directed towards the middle region of human RELA

RELA antibodyKey Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WB
Species Reactivity	BovineMousePigRat
Concentration	1mg/ml
Purification	Affinity purified

RELA antibodyTarget Information

Gene Symbol	RELA
Gene Synonyms	MGC131774 NFKB3 p65
Gene Full Name	V-rel reticuloendotheliosis viral oncogene homolog A (avian)
Gene Summary	p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
Alternative Names	MGC131774 NFKB3 p65
Molecular Weight(MW)	60kDa
Sequence	551 amino acids

RELA antibodyDatabase Links

Entrez Gene	5970
SwissProt ID	Q04206
Protein Accession	NP_068810

RELA antibodyApplication

Immunoblot

Lane1: 50 ug HCT116 cell lysate
Lane2: 50 ug HT-29 cell lysate
Lane3: 50 ug T47D cell lysate
Lane4: 50 ug HEK-293T cell lysate
Lane5: 50 ug MCF7 cell lysate
Lane6: 50 ug MDA-MB-231 cell lysate
Primary Antibody Dilution:
2 ug/ml
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
Gene Name:
RELA
Submitted by:
Anonymous
Western blot

0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
Western blot

RELA antibody - middle region validated by WB using HCT116/Ht-29/T47D/HEK-293T/MCF/MDA-MB-231 cell lines at 2ug/ml.

Additional Information

Form	Liquid
Storage Instructions	Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.