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產(chǎn)品名稱: c-Myc Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
c-Myc Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。c-Myc Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
c-Myc Antibody
的詳細(xì)介紹
c-Myc AntibodyProduct Profile
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Product Name
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c-Myc Antibody (C-19)
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Antibody Type
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Tags Antibodies
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Modification Notes
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c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
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c-Myc AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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IgG
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Host Species
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Rabbit
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Tested Applications
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WBIPIFIHCELISA
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recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline:
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Species Reactivity
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HumanMouseRat
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Concentration
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200 μg/ml
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Purification
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Affinity purified
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c-Myc AntibodyTarget Information
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Alternative Names
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epitope mapping within the C-terminus of c-Myc of human origin
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Tissue Specificity
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epitope mapping within the C-terminus of c-Myc of human origin
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c-Myc AntibodyDatabase Links
c-Myc AntibodyApplication
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Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
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Application
Western blot analysis of c-Myc expression in non-transfected: (A) and mouse c-Myc transfected: (B) 293T whole cell lysates.
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Application
Western blot analysis of c-Myc expression in Jurkat whole cell lysate.
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Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse uterus tissue showing nuclear localization.
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Application Notes
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recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
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Storage Buffer
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phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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