p53 (phospho Ser46) Antibody
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產(chǎn)品名稱: p53 (phospho Ser46) Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
p53 (phospho Ser46) Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。p53 (phospho Ser46) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
p53 (phospho Ser46) Antibody
的詳細(xì)介紹
p53 (phospho Ser46) AntibodyProduct Profile
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Product Name
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p53 (phospho Ser46) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes1) Dhavan, R. and Tsai, L. H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24 (35): 5431-5442. Mayo LD, et al. (2005) J Biol Chem. 280 (28): 25953-25959. Wang L, et al. (2005) Oncogene. 24 (18): 3020-3027.
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Immunogen
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p53 (Phospho-Ser46) antibody was raised against a peptide sequence around phosphorylation site of serine 46 (M-L-S (p) -P-D) derived from Human p53.
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p53 (phospho Ser46) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFWB
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Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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p53 (phospho Ser46) AntibodyTarget Information
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Gene Symbol
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TP53
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Alternative Names
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P53
BCC7
LFS1
TRP53
ERBB
ERBB1
HER1
Tumor suppressor p53
Phosphoprotein p53
Antigen NY-CO-13
TP53
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Molecular Weight(MW)
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53 kDa
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Tissue Specificity
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This antibody detects endogenous level of p53 only when phosphorylated at serine 46.
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p53 (phospho Ser46) AntibodyDatabase Links
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Entrez Gene
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7157
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Protein Accession
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NP_000537.3
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p53 (phospho Ser46) AntibodyApplication
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Application
Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Phospho-Ser46).
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Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with Anisomycin using p53 (Phospho-Ser46).
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Application Notes
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Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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