Product Name	RB1 Antibody
Antibody Type	Primary Antibodies
Product description	RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.1) Connell-Crowley,L., et.al., Mol. Biol. Cell(2), 287-301 (1997) More
Immunogen	This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	FACSIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	RB1
Alternative Names	Retinoblastoma-associated protein p105-Rb pRb Rb pp110 RB1 More
Molecular Weight(MW)	106 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Rat

Entrez Gene	5925
Protein Accession	P06400

Application

Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Western blot analysis of RB1 Antibody in 293 cell line lysates (35ug/lane)(2ug/ml)
Application

Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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