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產(chǎn)品名稱: Mouse Akt1 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Mouse Akt1 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Mouse Akt1 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Mouse Akt1 Antibody
的詳細(xì)介紹
Mouse Akt1 AntibodyProduct Profile
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Product Name
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Mouse Akt1 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Chang, Z., et al. Dev. Biol. 347(2):384-391(2010)
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Immunogen
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This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.
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Mouse Akt1 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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IHC-PWB
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Species Reactivity
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Mouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Mouse Akt1 AntibodyTarget Information
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Gene Symbol
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Akt1
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Alternative Names
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RAC-alpha serine/threonine-protein kinase
AKT1 kinase
Protein kinase B
PKB
Protein kinase B alpha
PKB alpha
Proto-oncogene c-Akt
RAC-PK-alpha
Thymoma viral proto-oncogene
Akt1
Akt
Rac
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Molecular Weight(MW)
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56 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Human
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Mouse Akt1 AntibodyDatabase Links
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Entrez Gene
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11651
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Protein Accession
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P31750
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Mouse Akt1 AntibodyApplication
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Application
Mouse Akt1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).
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Application Notes
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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